Table 2.
Overview of data and sample collection in NICE
| GW 18/28* |
GW 34 |
Birth | 48 h | 1 m | 2 m | 3 m | 4 m | 5 m | 6 m | 7 m | 8 m | 9 m | 10 m | 11 m | 12 m | 18 m | 48 m | |
| Questionnaires | ||||||||||||||||||
| ‘Allergic heredity and environment’† | X | |||||||||||||||||
| ‘Infant environment’ | X | |||||||||||||||||
| ‘Child environment’ | X | |||||||||||||||||
| ‘Infections and feeding’ | X | X | X | X | X | X | X | X | X | X | X | X | ||||||
| Dietary assessment | ||||||||||||||||||
| MealQ + ActiveQ, mothers | X | X | X | X‡ | ||||||||||||||
| MealQ + ActiveQ, father | X‡ | |||||||||||||||||
| TodMealQ + TodActiveQ, infant | X | |||||||||||||||||
| KidMealQ + KidActiveQ, infant | X | |||||||||||||||||
| Biological samples, mothers | ||||||||||||||||||
| Blood | X | X | X§ | X | X‡ | |||||||||||||
| Urine | X | X | ||||||||||||||||
| Saliva/Mouth swab | X | X | ||||||||||||||||
| Placenta | X | |||||||||||||||||
| Breast milk | X | X | ||||||||||||||||
| Biological samples, fathers | ||||||||||||||||||
| Blood | X | X‡ | ||||||||||||||||
| Urine | X | |||||||||||||||||
| Mouth swabs | X | |||||||||||||||||
| Biological samples, all children | ||||||||||||||||||
| Blood | X | X | X | X | X | |||||||||||||
| Urine | X | X | ||||||||||||||||
| Saliva | X | X | ||||||||||||||||
| Faeces | X | |||||||||||||||||
| Mouth swabs | X | X | X | X | X | |||||||||||||
| Meconium | X | |||||||||||||||||
| Hair | X | |||||||||||||||||
| Biological samples, only subcohort children | ||||||||||||||||||
| Blood | X | |||||||||||||||||
| Faeces | X¶ | X | X | X | ||||||||||||||
| Mouth swabs | X | |||||||||||||||||
| Clinical examination, children | ||||||||||||||||||
| RiNT/Spirometry | X | X | X | |||||||||||||||
| SPT | X | X | ||||||||||||||||
| Doctor’s diagnosis of allergy | X | X | ||||||||||||||||
| Neurological test | X | |||||||||||||||||
| Growth and obesity | X | |||||||||||||||||
| Oral health | X | |||||||||||||||||
All study-related visits, with the exception of the first sampling of mothers at gestational week 28 at the maternity clinics, will take place at the Sunderby Hospital. Midwives at the maternity ward will be responsible for all sampling at the birth timepoint and assist with collection of samples from the neonates at 48h. For all other time points post-48h the same clinical team, consisting of two specialized neonatal nurses, will meet all participating families. The same paediatric allergy specialist will examine all infants at 12 months and 48 months of age to diagnose any allergy and/or allergen sensitisation. At 48 months, the children will also undergo an assessment of neuropsychological development, motor skills assessment, (?) anthropometric measurements as well as an oral health examination.
*Questionnaires distributed at GW 18. Biological samples collected at GW 28.
†Questionnaires distributed to both mothers and fathers.
‡Blood collected from families where both parents and child are present.
§Blood samples are only collected from mothers in the subcohort.
¶Faeces samples are collected from children in the subcohort at week 1, 2, 4 and at month 4, 6 and 12.
GW, gestational week; h, hours; m, month; NICE, Nutritional impact on Immunological maturation during Childhood in relation to the Environment; RiNT, respiratory resistance with interruption technique; SPT, skin prick test.