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. 2018 Oct 8;2018:1650456. doi: 10.1155/2018/1650456

Figure 1.

Figure 1

Intermittent hypoxia increased RAGE expression and activates the NF-кB pathway in THP-1 monocytes. (a and b) RAGE expression of THP-1 cells exposed to IH compared to cells in normoxia over a period of 48 h. (c) Representative images of immunofluorescence staining showing the nuclear translocation of NF-кB p65 (green) in THP-1 cells. Scale bars indicate 10 μm. (d–f) Phosphorylated NF-κB p65 and IkBα protein expression and quantification of THP-1 cells exposed to IH compared with cells cultured under normoxia over a period of 48 h. (g–i) Phosphorylated NF-κB p65 and RAGE protein expression and quantification of NF-κB siRNA treated cells compared to untreated cells exposed to normoxia or IH. Data were represented as mean + SEM. ∗ represents significant difference compared with normoxic group, ∗/#p<.05, ∗∗p<.01, ∗∗∗p<.001.