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. 2017 Jul 18;3(2):153–160. doi: 10.1080/20961790.2017.1349639

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© 2017 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — RT-qPCR validation of 11 DEGs. (A) The transcript expression fold changes measured by RNA-seq and RT-qPCR are indicated by white and black columns, respectively. (A and B) The expression levels of nine up-regulated genes, Fosb, Atf3, IL6, Cxcl1, Zfp36, Jun, Rasd1, Fos and Apold1, two down-regulated genes, Sfrp2 and Fcna, were all consistent with the RNA-seq data. (B) The regression equation between RNA-seq (x) and RT-qPCR (y) data (Log₂ Ratio) is y = 0.860 7x − 0.268 9, while the R² is 0.978 3. (C) Those 11 DEGs showed no difference between abdominal skins of the injury group (RT-qPCR-A) and control group (RT-qPCR-B).