Fig. 2.

Cypj deficiency activates the NF-κB signaling in primary mBMM cells. a The strategy of CRISPR-Cas9 mediated genomic edition. A gene specific guide RNA (sgRNA) was used to achieve the targeting. b, c Percentage of mBMMs (b) and mBMDC (c) were analyzed by their specific lineage markers between WT and KO mice. d–i WT and Cypj-deficient primary mouse BMM cells were treated with or without LPS (100 ng ml⁻¹) (d, e), Tnf (20 ng ml⁻¹) (f, g), or HKLM (10⁷ CFU ml⁻¹) (h, i) for different times; Cypj abundance and the phosphorylation of the indicated proteins (d, f, h), as well as transcription of Tnf and Il-6 (e, g, i; N = 3) were detected. Error bars indicate S.D.; * p < 0.05, ** p < 0.01 (two-tailed Student’s t-test)