Fig. 4.

CYPJ binds to the NZF domain of TAB2 and TAB3. a Schematic diagram of FL and truncation variants of TAB2. b HA-CYPJ and Flag-tagged TAB2 fragments were co-transfected into 293T cells for co-IP assays to detect their interaction. c GST pull-down assays were performed using E.coli-expressed GST and GST-TAB2 truncations and lysates of 293T cells transfected with HA-CYPJ. # indicates the bands of GST-fused full-length and truncated TAB2. d E.coli-expressed GST and GST-fused NZF domains of TAB2, TAB3, and NEMO were used in GST pull-down assays with lysates of 293T cells transfected with HA-CYPJ or HA-CYPA. e E.coli-expressed GST and GST-fused NZF domains of LUBAC members, as well as TAB2 and TAB3 were used in GST pull-down assays with E.coli-expressed His-CYPJ. f ITC assays were performed to detect the binding of in vitro synthesized NZF peptides derived from TAB2 or TAB3 to prokaryotically expressed His-CYPJ