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. 2018 Oct 22;9(11):1076. doi: 10.1038/s41419-018-1117-5

Fig. 6. Knockout miR-10a in cancer GC by CRISPR/Cas9.

Fig. 6

a Schematic diagram showing the strategy to construct dox-inducible miR-10a knockout KGN cells. b–d Validation of the inducible CRISPR/Cas9 system in KGN cells. e Proliferation was repressed in miR-10a KO cancerous GC. f Apoptosis was induced in miR-10a KO cancerous GC. g, h MiR-10a KO promoted cisplatin-induced cell proliferation arrest and cell death in cancer GC. i The expression of PTEN-Akt/WNT pathway in miR-10a KO cancerous GC. j, k PTEN knockdown rescue proliferation and apoptosis in miR-10 KO cancerous GC. Each bar in the figure represents mean ± SEM of triplicates. *P < 0.05 and ** P < 0.01