Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 22;9:4373. doi: 10.1038/s41467-018-06878-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Molecular basis for the specific targeting of Nt-R by p62^ZZ. a A zoom-in view of the Nt-R degron binding site. b Superimposed ¹H,¹⁵N HSQC spectra of p62_{ZZ (120-190)} collected upon titration with the REEE peptide. Spectra are color coded according to the protein:peptide molar ratio. c, d Representative binding curves used to determine the K_d values by MST (c) or tyrosine fluorescence (d). Error bar in c represents s.d. in triplicate measurements. e Binding affinities of p62_ZZ for the indicated peptides measured by MST (^a, using p62_{ZZ (115-190)}) or tyrosine fluorescence (^b, using p62_{ZZ (120-171)}) or NMR (^c, using p62_{ZZ (115-190)}). Errors represent s.d. calculated from curve fitting (a, c) or duplicate midarguments (b). f–h Pulldown assays of wild-type or mutated p62-Flag expressed in HEK293 cells using X-nsP4 (f), X-BiP (g), and X-CRT (h) peptides. Uncropped blots are shown in Supplementary Figure 7. i A ribbon diagram of the structure of UBR-box of UBR2 (gray) in complex with Nt-R degron (blue, PDB 3NYN)