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. 2018 Oct 22;8:15575. doi: 10.1038/s41598-018-33964-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondria size for control, WT- and R495X-expressing neurons. (A) Images of mitochondria stained with MitoTracker (left) and merged images stained with anti-FLAG antibody (green), anti-TubulinβIII (blue) and MitoTracker (red) (right) for control (top), WT- (middle) and R495X-expressing neurons (bottom). Scale bar, 10 μm. (B) Magnified images of MitoTracker stained neuronal processes for control (top), WT- (middle) and R495X-expressing neurons (bottom). Scale bar, 5 μm. (C) Beeswarm plot for mitochondria major-axis length in control, WT- and R495X-expressing neurons. The number of analyzed mitochondria is indicated at the bottom. Red lines indicate the median, 25th and 75th percentiles. p < 0.0001, one-way ANOVA. ^**p < 0.01, post Tukey’s HSD test.