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. 2018 Oct 22;9(11):1082. doi: 10.1038/s41419-018-1116-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a After being starved with serum-free DMEM overnight, HN6 shNC and shPTK7 cells were treated with 100 ng/mL rhPOSTN for 0, 15, 30, 60, 120, 240 min. The lysates were prepared, and the expression of PTK7, p-Lrp6, Dvl2, Naked2, GSK3β, p-GSK3β, and β-Catenin was analyzed by western blot. Tubulin was used as a loading control. b After being starved with serum-free DMEM overnight, SCC-25 shNC and shPTK7 cells were treated with 100 ng/mL rhPOSTN for 0, 15, 30, 60, 120, and 240 min. The lysates were prepared, and the expression of PTK7, p-Lrp6, Dvl2, Naked2, GSK3β, p-GSK3β, and β-Catenin was analyzed by western blot. Tubulin was used as a loading control. c After being starved with serum-free DMEM overnight, CAL 27 Lv-NC and Lv-PTK7 cells were treated with 100 ng/mL rhPOSTN for 0, 15, 30, 60, 120, and 240 min. The lysates were prepared, and the expression of PTK7, p-Lrp6, Dvl2, Naked2, GSK3β, p-GSK3β, and β-Catenin was analyzed by western blot. Tubulin was used as a loading control. d After being starved with serum-free DMEM overnight, HN6 shNC and shPTK7 cells were treated with control DMEM medium or 100 ng/mL rhPOSTN for 30 min; the location of β-Catenin was detected by laser scanning microscopy. Scale bar: 10 μm