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. 2018 Oct 22;9(11):1081. doi: 10.1038/s41419-018-1125-5

Fig. 2. Prostate cancer cell lines are resistant to TL32711 + TNFα combination.

Fig. 2

a Western blot of RIPK1 and caspase-8 following caspase-8 immunoprecipitation in PC3, DU145 and VCaP cells treated for 3 h with 1 µM TL32711 in the presence and absence of 10 ng/mL TNFα; cells were pretreated with 20 µM z-VAD-fmk. b Annexin-V/Propidium Iodide flow cytometry analysis of PC3, DU145 and VCaP cells treated for 24 h 1 µM TL32711(TL) + /−10 ng/mL TNFα. c Cell viability assay in PC3, DU145 and VCaP cell lines treated for 72 h with 10 nM, 100 nM, 1 μM or 10 μM + /−10 ng/mL TNFα. d Cell Viability assay in PC3 and DU145 cells untransfected (UT) or pre-treated for 24 h with 10 or 1 nM Scrambled Control (SC1),cIAP-2, or XIAP siRNA followed by 48 h 1 μM TL32711 + 10 ng/mL TNFα. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001