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. 2017 Jan 20;19(1):43–53. doi: 10.1016/j.neo.2016.11.007

Figure 1.

Figure 1

Screening of GALNT6 substrates. (A) A pictorial schema for identification of GALNT6 substrates by using VVA-lectin pull down assay. (B) Silver staining for finally precipitated proteins and protein fraction eluted by GalNAc after VVA-lectin pull down using three HeLa cell-derived cell lines stably expressing empty vector (Mock), HA-tagged GALNT6 wild-type (WT), and enzyme activity-depleted GALNT6 (H271D). Differential protein bands indicated as arrows were excised and analyzed by mass spectrometry.