Figure 7.

GRP78 O-glycosylation is critical for cell survival under the nutrition deprivation condition. (A) MCF7-GRP78-WT and -alanine-substituted GRP78 stable cells were cultured in MEM with 10% FBS or after washed by PBS, and then in EBSS for additional 6 h, followed by western blot. Compared with MCF7-GRP78-WT cells, MCF7-alanine-substituted-GRP78 cells were more susceptible to apoptosis under the stress condition, showing higher level of apoptosis markers, such as cleaved PARP-1 and cleaved caspase 7. Nutrition deprivation treatment: EBSS medium treatment. (B) Graphs indicated protein band intensities of cleaved PARP-1 (left) or cleaved caspase 7 (right).