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O-glycosylation is critical for the interaction of GRP78 with PERK, IRE1α and ATF6. GALNT6 was knocked down by siRNA for 72 h in MCF7-GRP78-WT stable cells, and then Flag-tagged GRP78 was pulled down by using anti-Flag antibody followed by western blot. The binding ability of Flag-tagged GRP78 with endogenous PERK, IRE1α and ATF6 was significantly reduced after GALNT6 knockdown.
