Table 1.
Disease grade | 0 | 1 | 2 | 3 | 4 | Disease severity (%) | ||
---|---|---|---|---|---|---|---|---|
Treatment1 (V. dahliae+P. polymyxaShX301) | R1 | 48 | 2 | 4 | 6 | 2 | 15.30 | 13.50 ± 1.58 |
R2 | 50 | 1 | 4 | 5 | 2 | 12.90 | ||
R3 | 48 | 3 | 7 | 3 | 1 | 12.31 | ||
Treatment2 (V. dahliae) | R1 | 3 | 20 | 14 | 12 | 13 | 57.63 | 53.83 ± 1.67 |
R2 | 6 | 21 | 12 | 13 | 11 | 50.29 | ||
R3 | 5 | 21 | 10 | 14 | 13 | 53.57 | ||
Ck1 (P. polymyxaShX301) | R1 | 61 | 0 | 0 | 0 | 0 | 0.0 | 0.0 |
R2 | 61 | 0 | 0 | 0 | 0 | 0.0 | ||
R3 | 63 | 0 | 0 | 0 | 0 | 0.0 | ||
Ck2 (sterile water) | R1 | 62 | 0 | 0 | 0 | 0 | 0.0 | 0.0 |
R2 | 63 | 0 | 0 | 0 | 0 | 0.0 | ||
R3 | 61 | 0 | 0 | 0 | 0 | 0.0 |
ΔDisease severity was assessed for each plant on a 0 to 4 rating scale according to the percentage of foliage affected by acropetal chlorosis, necrosis, wilt, and/or defoliation (0 = healthy plant, 1 = 1 to 33%, 2 = 34 to 66%, 3 = 67 to 99%, 4 = dead plant)as described by Bejaranoalcazar et al. [3].
The disease assessment was carried out 45 days after planting for each plant on a 0 to 4 rating scale (0 = healthy plant, 1 = 1–33%, 2 = 34–66%, 3 = 67–99%, 4 = dead plant). Disease severity (%) = Σ (disease ratings × number of plants)/(maximum rating value × Total number of plants) × 100. R: repetition.