Fig. 5.

Evaluation of the time effect of celastrol treatment on cisplatin-induced acute kidney injury in mice.

(a) Representative images of periodic acid-Schiff staining (×200 and ×400) of kidneys. (b) Tubular injury score in mice. Data were presented as means ± SD of 10 random fields from each kidney. Blood urea nitrogen, serum creatinine (c) levels were measured. Data were presented as means ± SD (n = 6 in each group). (d) Western blots of NF-κB p65 and phospho-NF-κB p65 protein levels in kidney tissues. All experiments were duplicated for three times. Statistically significant differences were determined by one-way ANOVA, ### P < 0.001, *** P < 0.001. Pre: pretreatment; Sim: simultaneous treatment.