Fig 6.

Triple-fluorescent labelling marks oligodendrocytes with myelin-basic protein (A; MBP), cell nuclei with DAPI (B) and apoptosis with activated caspase-3 (C; AC3) in the white matter after 3 h ISO exposure. A merged image (D) shows a cell with a pyknotic nucleus co-labelled with AC3 and MBP, indicating the apoptotic cell is an oligodendrocyte. Triple-fluorescent labelling with glial fibrillary acidic protein (GFAP) to mark astrocytes (E) or Iba1 to mark microglia (F) display no co-labelling with AC3, suggesting neither cell type undergoes apoptosis.