Fig. 3.

Example of four-panel Odemis software interface of SECOM system with single and overlay channels. (A) Fluorescence light microscopy overview image of 100 nm thick ultrathin LR-white section containing Tupanvirus-infected cells. (B) Correlative light-electron microscopy overlay image of Tupanvirus particles (white arrows) located outside amoeba cell; n indicates amoeba nucleus. (C) Scanning electron microscopy of Tupanvirus particles depicted in (B). Depending on cutting plane of section, ultrastructural features such as capsid and tail can be seen. Mitochondria (asterisks) can be observed in amoeba cytoplasm. (D) Light microscopy of Tupanvirus particles (white arrows) depicted in (B) and detected by as fluorescent spots.