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Example of correlative light-electron microscopy overlay image of Tupanvirus-infected amoeba cells acquired with SECOM system. Nucleus (n) in cell as well as virus factories (asterisks) with multiplying viruses are shown. Tupanvirus particles can be found inside cells cytoplasm as well as inside cellular vacuoles or plasma membrane invaginations (solid and dashed thin arrows), probably on their way to externalization (thick arrow).
