Figure 3.

Therapeutic Effectiveness of Rluc-RFP-TK-FP-hAMSCs and Rluc-RFP-TK-hAMSCs Multiple Inoculations

(A) Representative BLI images of Pluc-GFP-U87 tumor-bearing mice: (top) control treated with GCV; (middle) treated with Rluc-RFP-TK-FP-hAMSCs plus GCV; (bottom) treated with Rluc-RFP-TK-hAMSCs plus GCV (n = 8 mice/group). Therapeutic cells were intra-tumorally inoculated every 3 weeks. BLI images were superimposed on black and white images. Arbitrary rainbow color scale depicts light intensity (red: highest; blue: lowest). (B) Graph showing Pluc-GFP-U87 bioluminescence activity values recorded in BLI images. Time points represent the median ± interquartile range. Significant differences for each day data were considered when *p < 0.05 by one-way ANOVA and Dunn’s multiple comparison post-test adjusted by Bonferroni method of the normalized data (log10 PHCs). (C) Kaplan-Meier plot showing animal survival throughout the experiment. Significant differences were considered when *p < 0.05 by log rank test adjusted by Bonferroni method.