Fig. 6.

Nrf2 and NF-κB pathways influence GSS expression. (A–C) RAW264.7 cells were pre-incubated with or without ML385 (5 μM) for 30 min or γ-GC (4 mM) and stimulated with LPS (1 μg/mL) for 12 h. (D–F) RAW264.7 cells were pre-incubated with or without Evo (5 μM) for 30 min or γ-GC (4 mM) and stimulated with LPS (1 μg/mL) for 12 h. (G–I) RAW264.7 cells were pre-incubated with or without Evo (5 μM) or Evo (5 μM) for 30 min or γ-GC (4 mM) and stimulated with LPS (1 μg/mL) for 12 h. Intracellular ROS levels, total GSH, and reduced GSH level were measured using the described methods. *P < 0.05, **P < 0.01, and ***P < 0.001; #P < 0.05 and ###P < 0.001. Data represent the mean ± SD of three independent experiments.