Figure 1.

Generation of Cardiomyocyte-Specific PRMT1-Deficient Mice

(A and B) X-gal staining of (A) fetal and (B) adult hearts in PRMT1^KI mice. The asterisk indicates cardiac artery.

(C) Western blotting of PRMT1 in neonatal mouse cardiomyocytes (CM) and non-myocytes (NM). Cardiac troponin I (cTnI) and vimentin were used as specific markers for CM and NM, respectively. GAPDH was used as loading control.

(D) Schematic diagram of Prmt1 gene targeting. Black rectangles indicate exons of Prmt1, and blue allows show primer set for genotyping.

(E) Representative images of genotyping in PRMT1-cKO mice.

(F) Western blotting analysis of PRMT1 in the hearts of control (ff/-) and PRMT1-cKO (ff/cre) mice. The hearts were harvested from 2-, 4-, and 6-week-old (shown as 2w, 4w, and 6w in the figure) mice.

(G) Representative images of cardiac sections stained with anti-PRMT1 antibody (green), Hoechst 33258 (blue), anti-cardiac troponin I antibody (magenta), and WGA (gray). Arrowheads indicate nuclei of cardiomyocytes. Arrows indicate non-cardiomyocytes.