Fig. 3.

HOTAIR mediated tumorigenicity through miR-122 downregulation in vivo.

HepG2 cells were infected with Lv-shHOTAIR or Lv-miR-122 injected subcutaneously into nude mice.

(a–d) Lv-shHOTAIR (a) and Lv-miR-122 (d) infected cells generated smaller tumors than their control cells. (b–e) the growth curves of tumor volumes were measured in the Lv-shHOTAIR (b) and Lv-miR-122 (e) group. (c–f) the tumor weights were measured in the Lv-shHOTAIR (c) and Lv-miR-122 (f) group. (g) miR-122 expression was increased in the xenograft tumors treated with Lv-shHOTAIR or Lv-miR-122. (h) the immunohistochemistry of Ki-67 stained sections followed by counterstaining with DAPI. Data are representative of three independent experiments and are presented as mean ± SD. *P < 0.05, **P < 0.01 (Student's t-test).