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. 2018 Sep 5;36:159–170. doi: 10.1016/j.ebiom.2018.08.055

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© 2018 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — HOTAIR regulated DNA methylation of miR-122 promoter region in HCC cells.

(a) a CpG island was located in the miR-122 promoter region of −5000 bp upstream of the miR-122 stem-loop. (b) the sequence level detail of the miR-122 promoter region (−4817 to −4526 bp upstream of the miR-122 stem-loop). (c) miR-122 promoter region was hypermethylated in HCC cells, but unmethylated in MIHA cells analyzed by MSP. (d) 5-Aza-dC treatment decreased the DNA methylation level of miR-122 promoter region in HCC cells analyzed by MSP. (e) 5-Aza-dC treatment increased the expression level of miR-122 in HCC cells. (f) knockdown of HOTAIR decreased DNA methylation level of miR-122 promoter region in HCC cells analyzed by BSP. Data are representative of three independent experiments and are presented as mean ± SD. *P < 0.05, **P < 0.01 (Student's t-test).