Fig. 6.

EZH2 mediated HOTAIR-induced DNMTs expression in HCC cells.

(a–e) knockdown of EZH2 by siEZH2-1 downregulated the expression levels of DNMT1, DNMT3A, and DNMT3B mRNA (a–c) and protein (d–e) in HCC cells. (f–j) siEZH2-1 reversed the upregulation of DNMTs mRNA (f–h) and protein (i–j) expression induced by HOTAIR in HCC cells. (k) siEZH2-1 abrogated the suppression of miR-122 induced by HOTAIR in HCC cells. Data are representative of three independent experiments and are presented as mean ± SD. *P < 0.05, **P < 0.01 (Student's t-test).