Fig. 7.

HOTAIR upregulated CCNG1 via repressing miR-122 in HCC cells.

(a–d) miR-122 suppressed whereas anti-miR-122 increased the expression levels of CCNG1 mRNA (a-b) and protein (c–d) in HCC cells. (e–h) CCNG1 expression was suppressed by siHOTAIR-1 whereas increased by pHOTAIR at both mRNA (e–f) and protein (g–h) levels in HCC cells. (i–l) either siEZH2-1, or siDNMT1, or AZA treatment, or miR-122 treatment reversed the enhanced expression of CCNG1 induced by HOTAIR at both mRNA (i–j) and protein (k–l) levels in HCC cells. Data are representative of three independent experiments and are presented as mean ± SD. *P < 0.05, **P < 0.01 (Student's t-test).