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. 2018 Oct 22;13(10):e0205306. doi: 10.1371/journal.pone.0205306

Fig 2. qPCR of selected CAV1-regulated mRNAs confirms up-regulation of ECM transcription.

Fig 2

A. Quantitative PCR was used to measure the levels of the transcripts shown in mRNAs purified from three different isolates of CAV1-/- MEFs (KO) and congenic WT controls. Expression fold change normalised to housekeeping controls is derived from ΔΔCT. The MEFs were grown in culture for the number of days shown. Bars are SD, N = 4 experimental repeats. P values were determined using a T-test. B. Quantitative PCR was used to measure the levels of the transcripts shown in mRNAs purified from CAV1-/- MEFs (KO) and congenic WT controls. The MEFs were from a single isolate per genotype, and were grown in culture for the number of days shown. Bars are SD, N = 4 experimental repeats. P values were determined using a T-test.