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. 2018 Oct 13;4(10):e00858. doi: 10.1016/j.heliyon.2018.e00858

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Characterisation of NUF2-mCherry expression. (A) Western blot using antibodies indicated on RIPA lysates from parental untargeted HCT116 cells as well as NUF2^{mCherry/mCherry} cells. For anti-NUF2 antibody, a non-specific band was observed, as indicated by *. Anti-Tubulin antibody signal used as a loading control; (B) Micrograph images showing fluorescence signal from live HCT116 and NUF2^{mCherry/mCherry} cells; (C) Confocal micrograph images showing fluorescence signals in NUF2^{mCherry/mCherry} cells. Nuclei were revealed using DAPI stain and used to distinguish interphase from mitotic cells.