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. 2018 Oct 13;4(10):e00858. doi: 10.1016/j.heliyon.2018.e00858

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Characterisation of NUF2-mCherry cell line proliferation and viability. (A) FACS scatter plot of HCT116 cells overlaid with NUF2^{mCherry/mCherry} cells. Numbers are percentages of live and single-gated events within gates indicated; (B) Histograms showing DRAQ5^TM staining profile of cell lines indicated. Populations divided into G₁, S and G₂/M. Numbers indicate percentage of live-gated cells; (C) Bar graphs showing percentage of cells at different cell cycle stages indicated. Exact p-values (n = 6, Mann-Whitney) are 0.0152 (G0/G1), 0.0043 (S) and 0.0022 (G2/M); (D) FACS plots of quadrant-gated HCT116 cells labelled with Muse^TM Annexin & Dead Cell Assay reagent for viability analysis. Quadrants indicated are live, early apoptotic, late apoptotic and dead; (E) Bar graphs showing percentage of cells in the different viability groups indicated. Exact p-values (n = 6, Mann-Whitney) are 0.9372 (live), 0.9372 (early apoptotic), 0.9372 (late apoptotic) and >0.999 (total apoptotic).