Fig. 2.

Technical validation of bioinformatics data of structural variation. (A) PCR strategy to verify the presence of miltefosine sensitivity locus (MSL) and their genes individually: LinJ.31.2370, LinJ.31.2380, LinJ.31.2390 and LinJ.31.2400. (B) Results of PCR reaction presented in “A”. The * highlights the L. infantum isolates from cured patients that showed mixed genetic profile for the presence of MSL, and were subsequently cloned. The ^ highlights the L. infantum isolates used as controls for the cloning process. The presence of the complete MSL in the L. infantum genome is demonstrated by a PCR product of ~14 kb, whilst loss is demonstrated by a ~1·2 kb DNA fragment.