Fig. 6.

T cell responses in A129 mice after pZIKV-3′UTR-∆20 immunization. Six-week-old A129 mice were immunized with pZIKV-3′UTR-Δ20 (0.5 μg) or DPBS (sham) using TriGrid™. On day 29 post-immunization, splenocytes were harvested for T cell analysis. (a) Total numbers of CD4⁺ T cell subsets per spleen. Splenocytes were cultured ex vivo with ZIKV for 24 h and stained for IFN-γ, TNF-α, and CD4 T cell markers. (b) Total numbers of CD8⁺ T cell subsets. Splenocytes were cultured ex vivo with ZIKV for 24 h (right panel) or with an E peptide for 5 h (left panel) and stained for IFN-γ, TNF-α, and CD8 T cell markers. Cytokines IL-2 (c), IFN-γ (d), and TNF-α (e) in cell culture media were measured after splenocytes were stimulated by ZIKV for 2 days. An unpaired nonparametric Mann-Whitney test was performed to analyze statistical significance.