Skip to main content
. 2018 Oct 4;5:1014–1020. doi: 10.1016/j.toxrep.2018.10.002

Fig. 4.

Fig. 4

The effect of dimethyl sulfoxide (DMSO) on glutathione content and generation of reactive oxygen species by using CellTracker Blue CMAC and dichlorofluorescein diacetate fluorescent stains, respectively. (A) Representative images of 3T3-L1 adipocytes exposed to various concentrations (0.1–100%) of DMSO for 1 h: an increased blue staining is equivalent to enhanced glutathione content. (B) Quantitative analysis of CellTracker Blue CMAC fluorescence staining. (C) Quantitative analysis of dichlorofluorescein diacetate fluorescence staining. Results are the mean ± SEM of three independent biological experiments relative to the experimental control (EC). ***P < 0.001 versus EC; ##p < 0.01, and ###p < 0.001 versus 0.01% DMSO.