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. 2018 Sep 27;36:281–292. doi: 10.1016/j.ebiom.2018.09.019

Fig. 6.

Fig. 6

CD44+/CD24+ antigens identify TRTICs and GSCs. a. Flow cytometry plot showing the expression of CD24 and CD44 in OSU53GSC and PKAC2GSC cells after 10 Gy radiation treatment (n = 3). b. Phase contrast and GFP+/CD24+/CD44+ GFP cells showing sphere-forming ability at day1 to day15. Microscopic images of OSU53GSC and OSU68GSC CD44+/CD24+ and CD44-/CD24- TRTICs 10 days after sorting pure population (n = 3). c. Kaplan-Meier survival plots of NOD-SCID mice bearing tumors from CD24+/CD44+ and CD24-/CD44- populations of OSU53GSC and OSU68GSC cells (n = 3) (log-rank (Mantel-Cox) test). d. Immunohistochemistry of Sox2, Nestin, CD44, Hif-1α and VEGF in U87 TRTIC-derived tumors (n = 3). The Hematoxylin and Eosin stained tumor section demonstrates the adjacent normal tissue in the Sox2, Nestin and CD44 images. The Hif-1α and VEGF adjacent normal tissue is represented as individual boxes to the left of their respective tumor staining. e. Western blots showing the protein expression pattern of unsorted, CD44+/CD24+ and CD24-/CD44- OSU53GSC and OSU68GSC cells (n = 3). Scale bar = 50 μm.