Fig. 1.

Schematic overview of the study design.

The rostral back of the mouse was depilated and each mouse was habituated in its own experimental home cage. Three days later, itch was induced by intradermal injections of histamine 0.8 μmol/50 μL, ST-1006 100 nmol/50 μL or 50 μL vehicle (PBS). Two hours later, the mice were anesthetized, perfused and coronal sections of the brain were taken. Every third section was stained for c-fos-DAB, every second analyzed for c-fos-EGFP.