Fig. 4.

c-fos + neuronal density in the medial habenula after intradermal injection of ST-1006, histamine or vehicle.

A./C. Representative single layer confocal c-fos-DAB or c-fos-EGFP images of the left medial habenula area (solid white line). Scale bar 50 μm. B./D. Total c-fos-DAB + or c-fos-EGFP + neurons in the medial habenula after intradermal injection of vehicle (50 μL), histamine (0.8 μmol/ 50 μL) or ST-1006 (100 nmol/ 50 μL). *p < 0.05, **p < 0.01, Kruskal-Wallis one-way ANOVA followed by Dunn’s post hoc test, single values with mean, c-fos density neurons/mm², n = 5–7 mice per group.