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. 2018 Sep 20;36:209–220. doi: 10.1016/j.ebiom.2018.09.006

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Characteristics of fibroblasts derived from patients with OSCC and isolation of exosomes. (A) Representative morphology of NFs and CAFs derived from patients with OSCC. Scale bar, 200 μm. (B) Western blotting analysis of the expression of CAF markers (α-SMA and FAP) and fibroblasts marker (vimentin) in isolated fibroblasts. (C) Immunofluorescence staining identification of CAFs using antibodies against vimentin, α-SMA, and FAP. Scale bar, 100 μm. (D) Transmission electron microscopy (TEM) showing exosomes isolated from NFs and CAFs-conditioned medium. Scale bar, 100 nm. (E) Western blotting analysis of the exosome marker CD63 and the Golgi matrix protein GM130 in exosome-enriched conditioned medium.