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. 2018 Sep 20;36:209–220. doi: 10.1016/j.ebiom.2018.09.006

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — MiR-34a-5p inhibits OSCC cell proliferation, migration, and invasion by targeting AXL. (A) AXL expression in OSCC cells transfected with AXL was examined by western blotting. (B) Colony formation in both AXL-transfected CAL27 and SCC15 cells was examined using a colony formation assay. *P < .05; **P < .01. (C) The growth rate of AXL-transfected CAL27 and SCC15 cells was examined using the CCK-8 assay. *P < .05; **P < .01. (D-E) The migration (D) and invasion (E) of AXL-transfected CAL27 and SCC15 cells was examined using a Transwell assay. **P < .01. (F-H) Clone formation (F), migration (G), and invasion (H) of CAL27 cells transfected with lenti-miR-control, lenti-miR-34a-5p, lenti-miR-34a-5p mock and lenti-miR-34a-5p AXL-OE was examined using a colony formation assay and Transwell assay, respectively. **P < .01.