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. 2018 Sep 20;36:209–220. doi: 10.1016/j.ebiom.2018.09.006

Fig. 6.

Fig. 6

MiR-34a-5p exerts its functions by inhibiting EMT and MMP-2/9 activation in OSCC cells. (A-B) The activation of β-catenin in CAL27 cells transfected with lenti-miR-control, lenti-miR-34a-5p, lenti-miR-34a-5p mock, and lenti-miR-34a-5p AXL-OE was examined using western blotting (A) and immunofluorescence staining (B); scale bar, 100 μm. (C) The effect of lenti-miR-control, lenti-miR-34a-5p, lenti-miR-34a-5p mock, and lenti-miR-34a-5p AXL-OE transfection in CAL27 cells on AKT/GSK-3β/β-catenin signaling pathway was examined using western blotting. (D) The effect of two target-specific AXL siRNAs transfection and AXL overexpression in CAL27 cells on the AKT/GSK-3β/β-catenin signaling pathway was examined using western blotting. (E-F) The expression of Snail was detected in CAL27 cells transfected with si-AXL (E) or si-β-catenin (F) was examined using real-time PCR and western blotting, respectively. **P < .01. (G-H) The activation of MMP-2 and MMP-9 in CAL27 cells transfected with the Snail siRNA and the vector containing full-length Snail in CAL27 was examined by real-time PCR (G) and Gelatin zymography (H). *P < .05; **P < .01. (I) Representative H&E and immunohistochemical staining for AXL, Snail, β-catenin, E-cadherin, vimentin, MMP-2 and MMP-9 in miR-34a-5p overexpression in SCC15 cells xenograft tumor tissue; scale bar, 200 μm. (J) Schematic representation of the contribution of miR-34a-5p-devoid exosomes derived from CAFs to EMT in OSCC cells via AKT/GSK-3β/β-catenin/Snail signaling cascade.