Figure 1.

PDX1 enrichment at CARE sites of stress and apoptosis genes. (A) Venn diagram demonstrating the overlap of RNAseq transcript profiles from Min6 cells deficient in PDX1, ATF4, or ATF5. (B) Percentage dysregulation direction of total regulated transcripts after shRNA mediated deficiency of Pdx1, Atf4, or Atf5 in Min6 cells. (C) p-values of top categories from gene ontology analysis of the subset of 149 genes commonly regulated by deficiency of Pdx1, Atf4, and Atf5. (D) PDX1 enrichment of CARE sites associated with Eif4ebp1, Trib3, and Ddit3 in mouse islet chromatin, demonstrated by ChIPseq tracks. (E) Calculation of PDX1 binding potential to CARE sites genome wide by peak strength and position related to PDX1 enrichment peaks.