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. 2018 Aug 1;17:39–48. doi: 10.1016/j.molmet.2018.07.007

Figure 5.

Figure 5

Gpt2 deficiency protects β cells from apoptosis due to stress. Min6 cells infected with lentivirus expressing shRNA targeting Gpt2 or non-targeting (NT) control, treated 96hrs post-infection with (A) PA (500uM) or vehicle control for 30 h, then harvested for protein and analyzed by western blot for cleaved caspase-3, GPT2, and tubulin. Quantitation of relative density for (B) cleaved caspase-3 or (C) GPT2. (D) Treatment with Tg (1uM) or vehicle control for 6 h, then harvested for protein and analyzed by western blot for cleaved caspase-3, GPT2, and tubulin. Quantitation of relative density for (E) cleaved caspase-3 or (F) GPT2. (G) Primary isolated mouse islets infected with lentivirus expressing GFP and shRNA targeting Gpt2 or non-targeting (NT) control, treated 48hrs post-infection with PA (500uM) + 25 mM glucose or control (Vehicle + 11 mM glucose). Stained for active caspase-3, dissociated into single cells and cytospun onto a slide. (H) Cell counts of caspase-3 positive/GFP positive cells. Bars show the mean and error bars the standard error of mean (S.E.M.) of 3 independent experiments. p-values were calculated with Student's T-Test (**, p < 0.01; compared to shNT + stress).