Figure 4.

Role of GL21.T/miR-34c on NSCLC Cell Proliferation

(A) Calu-1 cells were treated with GL21.T/miR-34c and GL21.T aptamer alone. Cell proliferation was analyzed by MTS assay 3, 4, 5, and 6 days upon transfection (left). p < 0.0001, compared to the non-transfected cells (Unt). Cells were treated with GL21.T/miR-34c and GL21.T aptamer alone and colony-formation assay was performed (right). (B) #5T (AXL⁺) or #9T (AXL⁻) cells were treated with GL21.T/miR-34c or GL21.T aptamer alone. Cell viability was analyzed by MTS assay. Bar graphs indicate mean value ± SD and the p value is calculated by using Student’s t test, p < 0.001 and < 0.0001, respectively, compared to the non-transfected cells (Unt). (C) Calu-1 cells were transfected with miR-34c-3p or treated with GL21.T aptamer or GL21.T/miR-34c conjugate. The cell migration capability was analyzed by transwell migration assay using 10% FBS as migration inducer. Migrated cells were stained with crystal violet and photographed. ****p < 0.0001, compared to the non-transfected cells (Unt). (D) Effect of GL21.T/miR-34c conjugate on long-term survival of Calu-1 cells after irradiation at different doses.