Subject Area	Biochemistry, Genetics and Molecular Biology
More specific subject area:	Developmental biology, zebrafish
Method name:	Zebrafish embryo, larvae dissociation to single cell suspension
Name and reference of original method	Our dissociation protocol was built empirically and optimized based on a method protocol from Gallardo et al. Gallardo VE, Behra M. Fluorescent activated cell sorting (FACS) combined with gene expression microarrays for transcription enrichment profiling of zebrafish lateral line cells. Methods. 2013;62(3):226-231.
Resource availability	Reagents: •Collagenase from Clostridium histolyticum (Sigma #C9891) •PBS 1X without Calcium and Magnesium (e.g. Corning #21-040-CV) •DMEM 1X with 4.5 g/L glucose, L-glutamine & sodium pyruvate (Corning 10-013-CV) •Fetal Bovine Serum (Fisher Scientific #SH30071.03HI) •0.25% trypsin-EDTA (Gibco #25200-056) •Pharmaceutical grade buffered Tricaine methane sulfonate (MS-222) Materials: •Heat block set at 30 °C •1.5 ml Microcentrifuge tubes (clear) •P1000 and P200 pipette tips •Centrifuge •Cell strainer, 70 μm mesh (e.g. FALCON #352350) •50 ml Conical tubes