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. 2018 Oct 15;10:4581–4590. doi: 10.2147/CMAR.S157858

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© 2018 Sun et al. This work is published and licensed by Dove Medical Press Limited

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(A) Results of luciferase activity assay confirm miR-365-induced suppressive action of the wild-type PSAT1 3′-UTR luciferase and not of the mutant 3′-UTR luciferase in human embryonic kidney-293 cells. (B) The abnormal expression of miR-365 in selected esophageal cell lines (EC109 and EC9706) suppressed the mRNA expression of PSAT1. (C) Western blot analysis for expression of PSAT1, E-cadherin, Snail, Vimentin, GSK-3β, and phosphorylated GSK-3β.

Note: *P<0.01 and **P<0.001.

Abbreviations: miR-365, miRNA-365; PSAT1, phosphoserine aminotransferase 1; 3′-UTR, 3′-untranslated region.