Figure 6.

Shugoshin Acts Downstream of Aurora B

(A and B) Contraction (A) and compaction (B) data in cells in the indicated cell-cycle stage expressing the indicated fusion proteins. CEN4^∗ and cen4− data from Figures 1E and 1D are shown for reference.

(C) Contraction of chr IV in anaphase cells expressing the indicated fusion proteins and of indicated genotypes. For reference, the data for ipl1-321, WT, and sgo1Δ, mutant cells are repeated from Figures 1E, 4D, and 5D, respectively.

(D) Compaction data for cells of indicated genotype in indicated cell-cycle stage. WT, H3S10D, and sgo1Δ data from Figures 4D and 5D, respectively, are shown as reference. Graphs, circles, and statistics are like those in Figure 1

(E) Profile of H3-pS10 levels from Figure 5E for the indicated region of chr IV in cells from the indicated genotypes.

(F) Changes in H3-pS10 levels between sgo1-TetR-mCh cen4− cells and SGO1 CEN4^∗ cells on each chromosome. Red dot, chr IV; black, other chromosome.

See also Figure S7.