Figure S7.

Microtubule Growth Episode Lengths in the Presence of DARPin and HSET Motor Speeds, Related to Figure 6

(A) Scatterplot depicting microtubule growth episode lengths at 60 μM tubulin in the presence of 2.9 μM DARPin (D1)₂. Number of growth trajectories measured – 158. Horizontal lines indicate the mean and the standard deviation. (B) Box-and-whiskers plot depicting the dependence of HSET-driven microtubule gliding speeds on the mCherry-HSET concentration used to immobilise the motor on the glass surface for gliding assays with GMPCPP-stabilized microtubules. These speeds agree with previously measured vertebrate kinesin-14 gliding speeds (Braun et al., 2017, Hentrich and Surrey, 2010). The boxes extend from 25^th to 75^th percentiles, the whiskers extend from 5^th to 95^th percentiles, and the mean value is plotted as a line in the middle of the box. Number of gliding episodes measured at different mCherry-HSET concentrations: 3.1 nM – 160, 12.5 nM – 102, 25 nM – 129, 100 nM – 108, 400 nM – 92. All experiments were carried out in self-organization buffer at 33°C.