Figure 1.

Quality Control of the Generated PCR Products and In Vitro-Transcribed mRNAs

ANG-1-, SDF-1α-, and VEGF-A-encoding mRNAs were synthesized and modified with 3′-PolyA₁₂₀ tail, 5′-ARCA, 100% m5CTP, 100% Ψ-UTP, and post-transcriptional phosphatase treatment. The specific lengths of the amplified DNA and the synthetic mRNA were detected using 1% agarose gel electrophoresis and GelRed staining at approximately 1.8 kb for ANG-1, 0.6 kb for SDF-1α, and 0.9 kb for VEGF-A. The 0.08- to 10-kb range mix DNA ladder and the 0.5- to 10-kb RNA ladder were used as length markers (Ms).