Fig. 4.

Ptpn22^−/−BMDC are able to promote Th1 responses. (A) Day 6 WT and Ptpn22^−/− LPS and OVA_323-339 pulsed BMDC were co-cultured with WT CD4⁺ OT-II T-cells for 6 days. At day 6, T-cells were restimulated with a fresh preparation of WT or Ptpn22^−/− BMDC for 48 h and then restimulated for 6 h with PMA, ionomycin and monensin and the proportion of CD3⁺ CD4⁺ IFNγ⁺ or IL-17⁺ cells was determined by intracellular flow cytometry. (B) Day 6 WT and Ptpn22^−/− LPS and OVA_323-339 pulsed BMDC were co-cultured with Ptpn22^−/− CD4⁺ OT-II T-cells for 6 days. At day 6 T-cells were restimulated with a fresh preparation of WT or Ptpn22^−/− BMDC and then restimulated for 6 h with PMA, ionomycin and monensin and the proportion of CD3⁺ CD4⁺ IFNγ⁺ or IL-17⁺ cells determined by intracellular flow cytometry. N = 6–7 per group with each data point representing an individual WT or Ptpn22^−/− BMDC preparation, points are paired by the OT-II responding T-cells. NS = not significant, *p ≤ 0.05, **p ≤ 0.01 determined by paired T-test.