Fig. 6.

Ptpn22^−/−BMDC promote Th1 responses in an LFA-1 dependent manner. (A) Day 6 WT and Ptpn22^−/− BMDC were pulsed for 24 h in the presence or absence of LPS. Cell surface expression of maturation markers was determined by flow cytometry. Median Fluorescent intensity (MFI) of CD86, CD40, MHCcII IA^b, and CD54 (ICAM-1). N = 6–10 independent experiments; bar represents mean ± s.e.m, NS = not significant determined by unpaired T-test. (B) Day 6 WT and Ptpn22^−/− LPS and OVA_323-339 pulsed BMDC were co-cultured with cell trace violet (CTV) labelled WT CD4⁺ OT-II T-cells for 6 days in the presence or absence of anti-LFA1 or isotype control. At day 6 cells were T-cells were restimulated with a fresh preparation of WT or Ptpn22^−/− BMDC for 48 h and then restimulated for 6 h with PMA, ionomycin and monensin and the proportion of CD3⁺ CD4⁺ IFNγ⁺ or TNFα⁺ cells was determined by intracellular flow cytometry. N = 4 independent experiments; bar represents mean ± s.d, NS = not significant, *p ≤ 0.05, determined by unpaired T-test.