Fig. 10.

Effect of the JNK/SAPK inhibitor (SP 600125), p38 MAPK inhibitor (PD 169316) and MEK inhibitor (U-0126) in the apoptosis induced by the complex cis-[PtCl(PIP-OH)(PPh₃)₂]PF₆ (CPP) in HL-60 cells determined by flow cytometry using Annexin V-FITC/PI staining. (A) Representative flow cytometric dot plots showing the percent of cells in the viable, early apoptotic, late apoptotic and necrotic stage. (B) Quantification of apoptotic HL-60 cells. For protection assay, the cells were pretreated for 2 h with 5 µM SP 600125, 5 µM PD 169316 or 5 µM U-0126, and then incubated with 4 µM CPP for 48 h. The negative control (CTL) was treated with the vehicle (0.1% DMSO) used for diluting the compound tested. Doxorubicin (DOX, 2 µM) was used as the positive control. Data are presented as the means ± S.E.M. of three independent experiments performed in duplicate. Ten thousand events were evaluated per experiment and cellular debris was omitted from the analysis. * P < 0.05 compared with the negative control by ANOVA, followed by the Student-Newman-Keuls test. # P < 0.05 compared with the respective treatment without inhibitor by ANOVA, followed by the Student-Newman-Keuls test.