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HPLC chromatograms of 16 reference standards separated with different Agilent RPLC columns with methanol and 0.1% formic acid as the mobile phase (254 nm). (A) Zorbax SB-C8 (4.6 mm × 150 mm, 5.0 μm); (B) Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm); (C) Eclipse plus C18 (2.1 mm × 100 mm, 3.5 μm); (D) Eclipse plus C18 (4.6 mm × 100 mm, 3.5 μm).
