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Isolation and characterization of BCSCs. A, Flow cytometry for analysis of CD24 and CD44 expression in two cell line CSCs (MDA‐MB‐231 and MCF‐7). B, qRT‐PCR analysis of CD24, CD44, ALDH1, Oct‐4, and Sox2 expression in all cell lines. C, The expression of ALDH1, Oct‐4, and Sox2 proteins was analyzed by western blot. D, Image of mammospheres generated by MDA‐MB‐231 and MCF‐7 CSCs at Day 7. Pictures were taken at 400× magnification
